Journal: NPJ Parkinson's Disease

Article Title: Dysfunctional digestive tract highlights the metabolic hallmarks of nanoplastic-exacerbated Parkinson’s pathology

doi: 10.1038/s41531-025-01145-2

Figure Lengend Snippet: a Representative HE-staining images of the upper duodenum in mice six months post-injection with PBS, PS nanoplastics or A53T αS. Scale bar: 400 μm. b Representative HE-staining images of the ileum in mice six months post-injection with PBS, PS nanoplastics or A53T αS. Scale bar: 200 μm. c TUNEL staining images of the ileum in mice. Scale bar: 400 μm. d Statistical analysis of ileum goblet cells per villus across different groups. e Statistical analysis of colon goblet cells per crypt across different groups. f Statistical analysis of ( c ). g Representative HE-staining images of the colon in mice six months post-injection with PBS, PS nanoplastics or A53T αS. Scale bar: 200 μm. h TUNEL staining images of the colon in mice. Scale bar: 200 μm. One-way ANOVA test was used between multiple comparisons, and unpaired two-tailed Student’s t -test was applied for comparison between two groups.

Article Snippet: Each section was covered with 50 μL TUNEL staining solution (Yeasen, China) and incubated at 37 °C in darkness for 1 h, followed by 3 PBS washes.

Techniques: Staining, Injection, TUNEL Assay, Two Tailed Test, Comparison